產品詳情
簡單介紹：
重組人類乳腺絲氨酸蛋白酶抑制劑蛋白與其它公司提供的重組蛋白不同，rHuMaspin蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類乳腺絲氨酸蛋白酶抑制劑蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類乳腺絲氨酸蛋白酶抑制劑蛋白
| Synonyms | SerpinB5, Protease Inhibitor 5 |
| Species | Human |
| Accession | P36952 |
| GeneID | 5268 |
| Source | 重組人類乳腺絲氨酸蛋白酶抑制劑蛋白Escherichia coli. |
| Molecular Weight | Approximately 42.1 kDa, a single non-glycosylated polypeptide chain containing 375 amino acid residues. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | 重組人類乳腺絲氨酸蛋白酶抑制劑蛋白MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED 重組人類乳腺絲氨酸蛋白酶抑制劑蛋白TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data is not available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuMaspin as determined by LAL method. |
| Reconstitution | 重組人類乳腺絲氨酸蛋白酶抑制劑蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類乳腺絲氨酸蛋白酶抑制劑蛋白 | |
| Reference |
1. Goulet B, Kennette W, Ablack A, et al. 2011. Lab Invest, 91: 1181-7. 2. Schwarz S, Ettl T, Kleinsasser N, et al. 2008. Oral Oncol, 44: 563-70. 3. Abd El-Wahed MM. 2005. J Egypt Natl Canc Inst, 17: 173-83. 4. Katakura H, Takenaka K, Nakagawa M, et al. 2006. Lung Cancer, 51: 323-8. |
| Background | Mammary serine protease inhibitor (Maspin), also known as serpin B5 is a non-inhibitory serpin that is encoded by the SERPINB5 gene in humans. The protein is expressed predominantly in normal mammary epithelial cells but at significantly reduced levels or absent in most breast carcinomas. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The maspin functions as tumor suppressor, blocking the growth, invasion, and metastatic properties of mammary tumors. This anti-tumor activity is achieved, in part, by the ability of maspin to inhibit angiogenesis and to preferentially promote apoptosis of tumor cells. |
